Products supplied by ReSyn Biosciences (Pty) Ltd are for the research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial purposes. Commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.
Polyethyleneimine polymer bead containing magnetite
> 10 µg phosphopeptide per mg support Bead size:
5-10 µm average
-1 suspension in 20% ethanol
2-functional microspheres for highly-specific phosphopeptide enrichment
2 Performance Superiority
Comparative assessment of MagReSyn™ TiO
2 for phosphopeptide enrichment vs a leading competitor.
32P labelled CHK1 tryptic digests were enriched for phosphopeptides and alnalyzed by LC/MS/MS.
2 showed exceptional specificity with 100% of identified peptides being phosphorylated, vs 78% for the competitor.
Phosphopeptide recovery was 78% using MagReSyn™ TiO
2 and 70% using competitor TiO
Protein phosphorylation plays a pivotal role in most cellular processes and is therefore the
most widely studied post-translational modification, with 30% of the proteome transiently
phosphorylated at any given time. However, the low-abundant nature of phosphopeptides,
the low stoichiometry of the modification, and the physico-chemical properties of
phosphorylated peptides make proteome-wide characterisation of phosphorylation a
Consequently, technologies than can specifically enrich phosphopeptides and are compatible
with mass spectrometric analyses are highly desired. MagReSyn™ TiO
2 microspheres allow extremely specific, reproducible enrichment of phosphopeptides from complex biological
samples/protein digests. The microspheres show selective affinity for phosphoserine (pSer),
phosphothreonine (pThr) and phosphotyrosine (pTyr) residues.
2 microspheres outperform competitor products, allowing excellent recovery (>80%) of bound phosphopeptides. Importantly, MagReSyn™ TiO
2 microspheres show 100% specificity for phosphopeptides while batch to batch reproducibility ensures confidence in
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